Also figuring in the 23-player list for the Jan 14-Feb 5 continental showpiece is Bruno Ecuele Manga, a defender with English Championship side Cardiff City, China-based striker Malick Evouna and Didier Ndong with Premier League strugglers Sunderland.
Gabon co-hosted the 2012 Nations Cup with Equatorial Guinea, making it into the quarter-finals.
The Panthers are drawn in Group A with Guinea-Bissau, four-time winners Cameroon and Burkina Faso,runners-up to Nigeria in 2013.
Gabon captain Aubameyang could lead his team as African Player of the Year should he retain his crown.
The 27-year-old French-born forward is on the shortlist with Leicester City and Algeria winger Riyad Mahrez and Liverpool and Senegal winger Sadio Mane with the winner announced on January 5.
Goalkeepers: Didier Ovono (KV Ostende/BEL), Yves Stéphane Bitséki Moto (CF Mounana/GAB), Anthony Mfa Mezui
Defenders: Lloyd Palun (Red Star/FRA), André BiyoghoPoko (Karabukspor/TUR), Aaron Appindangoye (Laval/FRA), Franck Perrin Obambou (Stade Mandji/GAB), Bruno Ecuélé Manga (Cardiff City/WAL), Yoann Wachter (Sedan/FRA), Johann Serge Obiang (Troyes/FRA), Benjamin Zé Ondo (Mosta/MAL)
Midfielders: Junior Serge Martinsson Ngouali (IF Brommapojkarna/SWE), Levy Clément Madinda (Nastic Tarragona/ESP), Guélor Kanga Kaku (Red StarBelgrade/SRB), Merlin Tandjigora (Meixian Hakka/CHN), Didier Ndong (Sunderland/ENG), Samson Mbingui (Raja Casablanca/MAR), Mario Lemina (Juventus/ITA)
Forwards: Pierre-Emerick Aubameyang (Borussia Dortmund/GER), Malick Evouna (Tianjin Teda/CHN), Denis Athanase Bouanga (Tours/FRA), Serge Kevyn Aboue Angoue (Uniao Leiria/POR), Cedric Ondo Biyoghe (CF Mounana/GAB)